Sort-of. IIRC DOS games that wanted to use more than 512KB(?) of memory had to use one of a series of particular high memory access drivers. HIGHMEM.SYS ("XMS"?), EMM386.SYS ("EMS"?), etc. You had to load these and other drivers (in particular sound and mouse drivers, antivirus programs, etc.) when booting up in your CONFIG.SYS and/or AUTOEXEC.BAT. These were referred to as 'TSR' (terminate and stay resident) programs. There were all sorts of tricks to get things working... LOADHIGH, etc. In the end, most games required one particular approach to high memory, and you had to have a CONFIG.SYS/AUTOEXEC.BAT that left enough base memory free as well. It was a huge hassle. In later days most programs used DOS4GW ... https://en.wikipedia.org/wiki/DOS/4G ... which made things a lot easier.
I was a 90s kid, and was totally head over heels for Origin Systems' games (Ultima, Wing Commander, etc). Par for course, I had a selection of DOS boot disks to set up the right operating environment for each game. Ultima VII featured a memory manager[0] based on 'Unreal Mode', which made getting it running even more precarious. The quote at the bottom of that link from the developer is really telling.
Didn't get the Ultima series so never experienced that one, but an Origin Systems game I loved was System Shock. Still unbeaten in my book for raw experience as an early FPS RPG... so damn scary. It had so much complexity to the plot, it really set the bar high. I don't think it was matched until 15-20 years later ... many far more popular games were pathetically linear, simplistic and mono-dimensional by comparison. Other early games which left a mark for me: Heretic (which for unknown reasons performed very smoothly on my graphics hardware - Oak?), Descent (first really 3D game, excellent gameplay and many overnight sessions with friends), Dune II (defined the RTS genre on PC), Mechwarrior 2 (strongly immersive and awesome experience with a proper joystick), X-Wing/Tie Fighter (strongly immersive). Later, Command and Conquer (first modern RTS), C&C2: A Bridge too Far (awesome top-down historic battles via modem). Ahh, these days why do all the games seem so rinse-and-repeat... I think the last one I played that really impressed was Shenzhen IO.
Then making a boot disk using 4DOS to manage the whole lot of it. As each game had its own idea of what being 'setup' looked like with different other TSRs (mouse, cd, etc).
