How? What type of 2FA are you talking about? Is there any that does this that many people use?
The only thing I know that does encryption with 2FA is https://keepass.info/plugins.html#otpkeyprov . But I highly doubt it has much usage. It's going to be annoying typing in a 2FA every time you decrypt your password database (I decrypt my password database maybe 10 times per day). More concerningly, if you press the button on your 2FA device (this is HOTP, which requires you to press a button to get a new code) too many times, or typo the 2FA too many times, you can permanently lose access to your database because the HOTP device will advance past the point that the database supports.
So yes, it's a choice that can be made, but it has very major downsides.
The only thing I know that does encryption with 2FA is https://keepass.info/plugins.html#otpkeyprov . But I highly doubt it has much usage. It's going to be annoying typing in a 2FA every time you decrypt your password database (I decrypt my password database maybe 10 times per day). More concerningly, if you press the button on your 2FA device (this is HOTP, which requires you to press a button to get a new code) too many times, or typo the 2FA too many times, you can permanently lose access to your database because the HOTP device will advance past the point that the database supports.
So yes, it's a choice that can be made, but it has very major downsides.