Off-targets is something you always look for. In a research setting, you'll usually be back-crossing to the parental line to clean up the background anytime you transform something.
My experience is in plants, so my point of reference is agrobacertium tumefaciens transformation (agro). To say that CRISPR is cleaner is a massive understatement. It takes a little doing, though, with some labs optimizing for particular species. Some of these optimized results have been astounding, often with no detectable off-site modifications, and 100% efficiency (no need to wait for the T2s for homozygotes)!
I'm not worried. I suppose it's good to point out that it's not ready for full-scale gene-therapy yet, but I'm not aware of anyone saying that it is.
My experience is in plants, so my point of reference is agrobacertium tumefaciens transformation (agro). To say that CRISPR is cleaner is a massive understatement. It takes a little doing, though, with some labs optimizing for particular species. Some of these optimized results have been astounding, often with no detectable off-site modifications, and 100% efficiency (no need to wait for the T2s for homozygotes)!
I'm not worried. I suppose it's good to point out that it's not ready for full-scale gene-therapy yet, but I'm not aware of anyone saying that it is.