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Here, follow the instructions to download and install the HDF5 plugin for Fiji:

http://lmb.informatik.uni-freiburg.de/resources/opensource/i...

From the same site you can download "pollen.h5". Then follow the steps to load the dataset in "hyperstack (multichannel)" mode. This will open a window where each frame in the movie is a z-slice of the 3D image.

Then go to Plugins/Volume Viewer (scroll down) and switch the mode (top left) to "Volume". (this is what you should get: http://i.imgur.com/0QoGa1t.png)

The same people also published lots of 3D data of the zebrafish embry. Have a look at:

http://vibez.informatik.uni-freiburg.de/

Look for "e098.h5" to download it.

If you have never worked with the HDF5 format before, you can use HDF5 View (https://support.hdfgroup.org/products/java/hdfview/) to look into the files. As you can see here (http://i.imgur.com/nnTiyQh.png) the data in pollen.h5 basically is a uint8-array of 193x199x419.

There are libraries for many languages to read HDF5. Eg. "h5py" for Python.



Thanks for the references, looking.

By the way, check out https://www.youtube.com/watch?v=-PVAcLlYUpg


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